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Our latest TechNote is here!

 
"Quick Characterization of Binding onto Unpurified GPCRs"
 

In this TechNote, we show how the WAVEsystem can be used for the kinetic analysis of G-protein binding onto detergent-solubilized, unpurified GPCRs. By shortening a typical 10-step purification process, this method requires significantly less material (30mL of cell culture) and less time (less than 1 hour), thereby offering an alternative for screening purposes at an early stage of the drug discovery process involving membrane proteins. 

   

 

 
 
  • Small molecule characterisation
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  • Fragment-Based Drug Discovery (FBDD)
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  • Kinetics of weak binders with fast off-rates
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  • Measurements in crude matrices
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  • Low immobilization kinetics avoiding mass transport
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  • Robustness against low-soluble, aggregating molecules 
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  • Kinetics of small molecules binding to solubilized GPCR
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  • Large Drug Targets
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  • Sample Recovery
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  • Kinetics and affinity of antibodies in crude serum
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For more information, please click on any of the Application Note links, or get in touch using our Contact page to discuss your specific needs.